2D Gel Electrophoresis

 

 Two-dimensional gel electrophoresis (2D-GE) is a powerful analytical technique used to separate complex protein mixtures based on isoelectric point (pI) and molecular weight. It combines isoelectric focusing (IEF) in the first dimension with SDS-PAGE in the second, allowing high-resolution protein profiling. This technique is essential in proteomics for detecting post-translational modifications, isoforms, and quantitative protein expression differences. The accuracy and reproducibility of 2D-GE depend on optimized workflows and reliable equipment. Companies like SERVA Electrophoresis (Germany) and Cleaver Scientific (UK) offer specialized instruments and reagents to support high-performance 2D electrophoresis. This bulletin outlines the 2D-GE workflow, required equipment, and specific SERVA product recommendations for efficient IEF and SDS-PAGE execution

Overview

SERVA HPE BlueHorizon System

 

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Serva 2D Technical Notes

A horizontal electrophoresis system for flatbed gels and IPG strips

HPE™ BlueHorizon™ horizontal electrophoresis system is equipped with high efficiency chiller and a high voltage BluePower™ IPG power supplies (e.g. 6000 IPG, 3000 HPE) required for high-voltage focusing i

The system is designed to perform a wide range of electrophoresis techniques, including IEF of IPG strips, gel electrophoresis, SDS-PAGE, native PAGE, and SSCP. Its robust construction ensures high reproducibility and repeatability of results, making it ideal for research laboratories, quality testing facilities, and diagnostic applications. With precision control and reliable performance, this system supports consistent and accurate protein and nucleic acid separation across diverse experimental needs.

Smart Design – Made in Germany

HPE™ BlueHorizon™ is produced in Germany from high quality materials. Choose a life-time chamber that delivers reproducible results.

High efficient cooling plate

Its sophisticated materials, our know-how and the exploration of new possibilities allows an uniform and continuous heat dissipation.

Platinum electrodes

The electrodes of the HPE™ BlueHorizon™ are made of platinum coated titanium rods – indestructible and always lying flat.

Stackable

Up to four HPE™ BlueHorizon™ can be stacked and connected to one chiller and one power supply. Just order stabilizing feet and clamps – installation is easy.

 

Contact:  technical@genaxy.com for more details

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